Dieta de queixadas (Tayassu pecari) por DNA metabarcoding de fezes na região do Parque Nacional das Emas (Goiás, Brasil)
Palavras-chave:
Conflito humano-fauna. Conservação. Espécie ameaçada. Manejo do fogo.Resumo
Tayassu pecari é uma espécie ameaçada de extinção que tem o Parque Nacional das Emas como importante refúgio no Cerrado. Nesta região, T. pecari é identificada como espécie-problema devido aos danos causados às plantações, em especial ao cultivo de milho. Para identificar a constituição da dieta dos queixadas, este trabalho utilizou DNA metabarcoding em fezes. Foram encontradas 80 espécies de plantas em 47 famílias, em 16 amostras de fezes utilizando o barcode trnL. Foi observado que existe uma constituição alimentar distinta para cada ponto de coleta e que a diversidade na dieta é maior nas amostras de dentro do Parque. Destaca-se a presença de soja, cana de açúcar, brachiaria e, em grande proporção, milho. Os resultados permitiram identificar 21 espécies que podem responder positivamente ao fogo, seja em nível de gênero ou de família, indicando que queimas prescritas na época correta podem aumentar a disponibilidade de alimentos para os queixadas no interior do Parque.
Referências
Altrichter, M., Taber, A., Beck, H., Reyna-Hurtado, R., Lizarraga, L., Keuroghlian, A., & Sanderson, E. W. (2012). Range-wide declines of a key Neotropical ecosystem architect, the Near Threatened white-lipped peccary Tayassu pecari. Oryx, 46(1), 87–98
Aranda, M. (1994). Importancia de los pecaries (Tayassu sp) en la alimentacion del jaguar (Panthera onca). Acta Zoológica Mexicana, 62, 11-22
Barreto, G., Hernandez, O., & Ojasti, J. (1997). Diet of peccaries (Tayassu tajacu and T. pecari) in a dry forest of Venezuela. Journal of Zoology, 241, 279-284
Batalha, M. A., & Martins, F. R. (2007). The Vascular Flora of the Cerrado in Emas National Park (Central Brazil): a Savanna Flora Summarized. Brazilian. Archives of Biology and Technology, 50(2), 269-277
Bradley B. J., Stiller M., Doran-Sheehy D. M., Harris T., Chapman C. A., Vigilant L., & Poinar H. (2007). Plant DNA sequences from feces: potential means for assessing diets of wild primates. American Journal of Primatology, 69(6), 699-705
Callahan, B.J., Mcmurdie, P.J., Rosen, M.J., Han, A.W., Johnson, A.J., & Holmes, S.P. (2016). DADA2: High-resolution sample inference from Illumina amplicon data. Nature Methods, 13(7), 581-583
Desbiez, A. L. J., Bodmer, R. E., & Tomas, W. M. (2010). Mammalian densities in a Neotropical wetland subject to extreme climatic events. Biotropica, 42(3), 372–378
Desbiez, A. L. J., Santos, S., Keuroghlian, A., & Bodmer, R. (2009). Niche Partitioning Among White-Lipped Peccaries (Tayassu pecari), Collared Peccaries (Pecari tajacu), and Feral Pigs (Sus Scrofa). Journal of Mammalogy 90, 119-128
Elston, J. J., Klinksiek, E. A., & Hewitt, D. G. (2005). Digestive Efficiency of Collared Peccaries and Wild Pigs. The Southwestern Naturalist, 50(4), 515–519
Evans, D.M., Kitson, J.J.N., Lunt, D.H., Straw, N.A., & Pocock, M.J.O. (2016). Merging DNA metabarcoding and ecological network analysis to understand and build resilient terrestrial ecosystems. Functional Ecology, 30, 1904-1916
Flora do Brasil 2020. (2022 oct 25) Jardim Botânico do Rio de Janeiro. http://floradobrasil.jbrj.gobr/
Galetti, M., Camargo, H., Siqueira, T., Keuroghlian, A., Donatti, C., Luisa, M., Jorge, M. L. Pedrosa, F., Kanda, C. Z., & Ribeiro M.C. (2015) Diet Overlap and Foraging Activity between Feral Pigs and Native Peccaries in the Pantanal. Plos One, 10(11), e0141459
Garshelis, D., Baruch-Mordo, S., Bryant, A., Gunther, K., & Jerina, K. (2017). Is diversionary feeding an effective tool for reducing human–bear conflicts? Case studies from North America and Europe. Ursus, 28, 31-55
Hollingsworth, P.M., Graham, S.W., & Little, D.P. (2011) Choosing and Using a Plant DNA Barcode. Plos One, 6(5), e19254
IBAMA/MMA. (2021) Plano de Manejo do Parque Nacional das Emas. https://www.icmbio.gobr/portal/images/stories/imgs-unidades-coservacao/parna_emas.pdf.
Jácomo, A., Furtado, M., Kashivakura, C., Marinho-Filho, J., Sollmann, R., Torres, N., & Silveira, L. (2013). White-lipped peccary home-range size in a protected area and farmland in the central Brazilian grasslands. Journal of Mammalogy, 94, 137-145
Jorge, M.L.S.P., Keuroghlian, A., Bradham, J., Oshima, J.E.F., Ribeiro, M.C. (2019). White-Lipped Peccary Movement and Range in Agricultural Lands of Central Brazil. In: Reyna-Hurtado, R., Chapman, C. (eds) Movement Ecology of Neotropical Forest Mammals. Springer, Cham. https://doi.org/10.1007/978-3-030-03463-4_4
Jorge, MLSP, Bradham, JL, Keuroghlian, A, Oshima, JEF, Ribeiro, MC. (2021) Permeability of Neotropical agricultural lands to a key native ungulate—Are well-connected forests important?. Biotropica. 53: 201– 212
Keuroghlian, A., Desbiez, A., Reyna-Hurtado, R., Altrichter, M., Beck, H., Taber, A., & Fragoso, J.M., (2022 apr 18) Tayassu pecari. The IUCN Red List of Threatened Species https://www.iucnredlist.org/species/41778/44051115
Keuroghlian, A., Léonard, A., Desbiez, J., De Mello Beisiegel, B., Medici, E. P., Gatti, A., Rossano, A., Pontes, M., Bueno De Campos, C., Farah De Tófoli, C., Amorim, E., Junior, M., Cavalcanti De Azevedo, F., Medeiros De Pinho, G., Luís, J., Cordeiro, P., Da, T., Santos Júnior, S., Aparecida De Morais, A., Mangini, P. R., Flesher, K., Rodrigues, L. F., Bonjorne De Almeida, L., Barber, R. E (2022 apr 5). Avaliação do Risco de Extinção do Queixada Tayassu pecari Link, 1795, no Brasil. Ano II, 1, 3–11, 2012. http://www.icmbio.gobr/portal/images/stories/biodiversidade/fauna-brasileira/avaliacao-do-risco/ungulados/Tayassu_pecari_queixada.pdf
Kiltie, R.A., & Terborgh, J. (1983). Observation on the behavior of Rain Forest peccaries in Peru: why do White-lipped peccaries form heds? Zeitschrift fur Tierpsychologie, 62, 241-255
Klink, C. A., & Machado, R.B.. (2005). Conservation of the Brazilian Cerrado. Conservation Biology 19, 707–713
Lima, M., Augusto, C.A., Ilan, M.I., Junior, C.A.S., Costa, G.M., & Santos, R.C. (2019) The paradoxical situation of the white-lipped peccary (Tayassu pecari) in the state of Mato Grosso, Brazil. Perspectives in Ecology and Conservation, 17, 36–39
Mallott, E. Garber P.A., & Malhi R.S. (2018). trnL outperforms rbcL as a DNA metabarcoding marker when compared with the observed plant component of the diet of wild white-faced capuchins (Cebus capucinus, Primates). Plos One, 13(6), e0199556
Maravalhas, J., & Vasconcelos, H.L. (2014). Revisiting the pyrodiversity-biodiversity hypothesis: long-term fire regimes and the structure of ant communities in a Neotropical savanna hotspot. Journal of Applied Ecology, 51, 1661–1668
Martin, M. (2011). Cutadapt removes adapter sequences from high-throughput sequencing reads. EMBnet Journal, 17(1), 10–12
Massei, G., Roy, S., & Bunting, R. (2011). Too many hogs? A review of methods to mitigate impact by wild boar and feral hogs. Human-Wildlife Interactions, 5(1), 79–99
Mcmurdie, P.J., & Holmes, S. (2013). phyloseq: an R package for reproducible interactive analysis and graphics of microbiome census data. Plos One, 8(4), e61217
Oksanen, J., Blanchet, F.G., Friendly, M., Kindt, R., Legendre, P., Mcglinn, D., Minchin, P.R., O'hara, R.B., Simpson, G.L., Solymos, P., Stevens, M.H.H., Szoecs, E., & Wagner, H. (2018). vegan: Community Ecology Package. Disponível em https://CRAN.R-project.org/package=vegan
Paviolo, A., De Angelo, C., Di Blanco, Y., Ferrari ,C., Di Bitetti, M., Kasper, C.B., Mazim, F.D., Soares, J.B.G., & Oliveira T.G.(2006) The Need of Transboundary Efforts to Preserve the Southernmost Jaguar Population in the World. Cat News, 45, 12-14
Pilon, N.A.L., Hoffmann, W.A., Abreu, R.C.R., & Duringan, G. (2018) Quantifying the short-term flowering after fire in some plant communities of a cerrado grassland. Plant Ecology & Diversity, 11(3), 259-266
Pilon, N.A.L., Cava, M.G.B., Hoffmann, W.A., Abreu, R.C.R., Fidelis, A., & Durigan, G. (2021) The diversity of post‐fire regeneration strategies in the cerrado ground layer. Journal of Ecology, 109(1), 154-166
Pivello, R. (2011). The use of fire in the Cerrado and Amazonian Rainforest of Brazil: Past and Present Fire. Ecology 7(1), 24-39
Painkow Neto, E. Uso e Seleção de Hábitats por Queixadas (Tayassu pecari) em Paisagem Agrícola na Região Sul do Parque Nacional Das Emas. 2019. 16p Dissertação (Mestrado Profissional em Conservação da Biodiversidade e Desenvolvimento Sustentável) - Instituto de Pesquisas Ecológicas, Nazaré Paulista, 2019.
Pompanon F., Coissac E., & Taberlet P. (2011). Metabarcoding, a new way to analyze biodiversity Biofutur, 319, 30-32
Pompanon F., Deagle B.E., Symondson W.O.C., Brown D.S., Jarman S.N., & Taberlet P. (2012). Who is eating what: Diet assessment using next generation sequencing. Molecular Ecology, 21(8), 1931–1950
POWO, Plants of the World Online. (2021) Facilitated by the Royal Botanic Gardens, Kew. Disponível em: http://www.plantsoftheworldonline.org/
R Core Team (2020). R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. https://www.R-project.org/.
Ramos-Neto, M. B., & Pivello, R. (2000). Lightning Fires in a Brazilian Savanna National Park: Rethinking Management Strategies. Environmental Management, 26(6), 675–684
Redford, K. H. (1985). Emas National Park and the plight of the Brazilian cerrados. Oryx, 9(4), 210–214
Riaz T., Shehzad W., Viari A., Pompanon F., Taberlet P., & Coissac E. (2011) EcoPrimers: inference of new DNA barcode markers from whole genome sequence analysis. Nucleic Acids Research, 39(21), e145
Robeson, M., Khanipov, K., Golovko, G., Wisely, S., White, M., Bodenchuck, M., Smyser, T., Fofanov, Y., Fierer, N., & Piaggio, A. Assessing the utility of metabarcoding for diet analyses of the omnivorous wild pig (Sus scrofa). Ecology and Evolution, 8(1), 185-196.
Taber, A., Chalukian, S., Altrichter, M., Minkowski, K., Lizarraga, L., Sanderson, E., Rumiz, D., Edsel, A., Angelo, C., Antunez, M., Ayala, G., Beck, H., Bodmer, R., Salvador, B., Cartes, J., De Bustos, S., Eaton, D., Emmons, L. , Estrada, N., & Zapata-Ríos, G. El Destino de los Arquitectos de los Bosques Neotropicales: Evaluacion de la Distribucion y el Estado de Conservacion de los Pecaries Labiados y los Tapires de Tierras Bajas. Pigs, Peccaries and Hippos Specialist Group (IUCN/SSC), Tapir Specalist Group (IUCN/SSC), Wildlife Conservation Society, and Wildlife Trust. 2008
Poinar, H.N., Hofreiter, M., Spaulding, W.G., Martin, P.S., Stankiewicz, B.A., Bland, H., Evershed, R.P., Possnert, G., & Pääbo S. (1998). Molecular coproscopy: dung and diet of the extinct ground sloth Nothrotheriops shastensis. Science, 281(5375), 402-406
Soininen, E., Valentini, A., Coissac, É., Miquel, C., Gielly, L., Brochmann, C., Brysting, A., Sønstebø, J. H., Ims, R., Yoccoz, N., & Taberlet, P. (2009). Analyzing diet of small herbivores: The efficiency of DNA barcoding coupled with high-throughput pyrosequencing for deciphering the composition of complex plant mixtures. Frontiers in Zoology. 6, 16
Taberlet, P., Coissac, E., Pompanon, F., Gielly, L., Miquel, C., Valentini, A., Vermat, T., Corthier, G., Brochmann, C., & Willerslev, E. (2007). Power and limitations of the chloroplast trnL (UAA) intron for plant DNA barcoding. Nucleic Acids Research, 35(3), e14
Tang Y, Horikoshi M, & Li W. (2016). ggfortify: Unified Interface to Visualize Statistical Result of Popular R Packages. The R Journal, 8(2), 474–485
Valentini, A., Pompanon F., & Taberlet, P. (2008). DNA barcoding for ecologists. Trends in Ecology & Evolution, 24(2), 110-117
Valentini, A., Miquel, C., Nawaz, M.A., Bellemain, E., Coissac, E., Pompanon, F., Gielly, L., Cruaud, C., Nascetti, G., Wincker, P., Swenson, J.E., & Taberlet, P. (2009). New perspectives in diet analysis based on DNA barcoding and parallel pyrosequencing: the trnL approach. Molecular Ecology Resources, 9(1), 51-60
Wang, Q., Garrity, G.M., Tiedje, J.M., & Cole, J.R. (2007). Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy. Applied Environmental Microbiology, 73(16), 5261-7526